STUDY ON RALASTONIA SOLANACEARUM CAUSING BACTERIAL WILT OF TOMATO AND ITS MANAGEMENT BY USING ANTAGONISTIC BACTERIA

Abstract

The experiment was conducted in the Disease Diagnostic Laboratory and Net house of Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka during the period of July 2012-June 2013 to find out suitable antagonistic bacteria against Ralstonia solanacearum causing bacterial wilt of tomato. Fourteen probable antagonistic bacterial isolates were isolated from rhizosphere soil of tomato and 6 isolates of Rhizobium leguminosorum collected from the department. In-vitro screening of these bacteria were done against R. solanacearum to detect the promising antagonistic bacteria. Ralstonia solanacearum was isolated from the wilt infected part of tomato plant and it’s morphological, biochemical and cultural features were studied. The bacterium was gram negative, rod shaped and showed positive results in KOH solubility test, starch hydrolysis test, catalase test, oxidase test, citrate utilization test, motility indole urease agar 0 C on TTC medium. Out of all antagonistic bacterial isolates, Pseudomonas fluorescens ...