DEVELOPMENT OF PLANT REGENERATION PROTOCOL FROM TUE EXPLANTS OF MESTA ('Hibiscus sabdariffa L.)

Abstract

An experiment was conducted in the Genetic Engineering Laboratory of the Cvtogeneties Department, Bangladesh Jute Research Institute (BJRI), Dhaka during the period from Januan to July 2006. Cotyledons (with attached petioles of mesta (Hibiscus sabdar?fth L.) were used as explant to investigate their in vitro re2eneration potentiality. Seed germination percentage was found highest on clinical cotton supported MS liquid medium (901/-0') than agar-based MS solidified medium (81.33%). Amone the combination. MS medium + SAP (2.0 m1) + IAA (0.5 mg/I) showed the highest shoot regeneration (58.33). No shoot regeneration was observed in MS media without hormone (NISO). Maximum shoot multiptication (46.67%) •as thund from shoot apex on XIS medium - BAP (2.0 m.1) - IAA (0.5 nw/I). MS medium with hormone and MS medium nithout hormone (MSO) were used for root formation from shoots. The highest number of initial roots per shoot (6.671 as found in the presence of 1AA (2.0 mg 1. [he maximum weight of roots per shoot (0.21 20 was obtained in IAA (1.0 mg 1. The highest number of' days to root initiation (9.00) was required in. IAA (0.5 mgb and the lowest (5.00) in IAA (0.0 mg'l) containing MS medium. The regenerated plantlets of mesta were successfully in soil.