ISOLATION AND IDENTIFICATION OF MYCOFLORA FROM MUSHROOM SUBSTRATES AND EVALUATION OF THEIR EFFICACY AGAINST THREE SELECTED PATHOGENIC FUNGI

Abstract

The study was carried out at the Molecular Plant Pathology Laboratory, Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, (SAU), Dhaka-1207, during the period of January to June, 2017 to isolate the mycoflora from different mushroom substrates and to evaluate their biological activities against selected pathogens (Fusarium oxysporum, Sclerotium rolfsii, Colletotrichum corchori). For this purpose three types of mushroom substrates viz. sawdust, rice straw and newspaper were used. Four fungi were isolated and identified from mushroom substrates. These were Aspergillus flavus, Aspergillus niger, Trichoderma harzianum, and Penicillium sp. In in-vitro dual culture method Trichoderma harzianum showed the best performance in reducing radial mycelial growth of the selected pathogenic fungi, whereas Penicillium sp showed the least significant result against these pathogens. T. harzianum showed the highest inhibition in case of Fusarium oxysporum (71.62%) followed by Sclerotium rolfsii (61.64%) and Colletotrichum corchori (57%).