IN VITRO REGENERTION OF TEA [Camellia sinensis (L.) O. Kuntze]

Abstract

The present research was carried out at Biotechnology Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207 from the period of January 2020 to September 2021 to evaluate the effect of different plant growth regulators on in vitro callus induction, shoot regeneration and root formation for Tea. The experiment was conducted at Completely Randomized Design (CRD) with 3 replications. Four levels of BA (1.00, 2.00, 3.00 and 4.00 mg/L) alone and in combination with four levels of NAA (1.00, 2.00, 3.00 and 4.00mg/L) were used for shoot induction. The treatment 2, 4-D was used for callus induction. Different concentration of IBA (300, 400 and 500 mg/L) was used with different dipping time for root induction of regenerated shoot in natural open condition. The highest percentage (86.51%) of callus induction was observed in combined treatment of 2.00 mg/L BA + 1.50 mg/L 2, 4- D with minimum (2.12) weeks. Highest percentage (84.39%) of shoot induction was recorded with 2.00 mg/L BA+ 2.00 mg/L NAA as well as in minimum (32.43) days. Maximum number of shoot (1.42, 2.46 and 4.49) and the highest length of shoot (1.41, 3.28 and 4.61) at 60, 90 and 120 DAI were recorded from the treatment 2.00 mg/L BA + 2.00 mg/L NAA. The highest percentage (75.32%) of root induction with minimum (6.02) weeks was recorded when regenerated shoots were dipped in 500 mg/L IBA solution for 30 minutes. The highest number of roots (4.20 and 7.80) and highest length of roots (3.31 and 5.80 cm) were recorded in 500 mg/L IBA concentration in 30 minutes dipping time. No root induction was recorded in controlled treatment. The protocol developed from the present study might be useful for large scale production of healthy, disease free planting material for Tea.