FIELD OBSERVATION OF SELECTED MUNGBEAN CULTIVARS AGAINST Mungbean Yellow Mosaic Virus (MYMV) AND ITS MOLECULAR DETECTION

Abstract

ABSTRACT

An experiment was conducted in field and laboratory condition under the Department of Plant Pathology of Sher-e-Bangla Agricultural University, Dhaka-1207 during 2020-2021. The field experiment was carried out in Randomized Complete Block Design (RCBD) with three replications to observe the field performances of ten selected mungbean cultivars and molecular study was done in Molecular Biology and Plant Virology Laboratory. From the field experiment results, it was revealed that three varieties namely BARI mung-7, BARI mung-8 and BINAmoog-9 showed moderately resistance and lower disease severity against Mungbean yellow mosaic virus (MYMV). Among the remaining selected varieties, four varieties namely BARI mung-5, BARI mung-6, BINAmoog-5 and BINAmoog-8 showed moderately susceptible whereas BU mug1, Sonamug and Chaitamug appeared as susceptible to highly susceptible and higher disease severity against MYMV. Field study results were approved by the molecular study through PCR test. All the tested varieties remained PCR positive which revealed that every single variety was MYMV infected. Different growth parameters and yield contributing characters were also studied. Among the tested varieties, the tallest plant found in BARI mung-7 and the shortest was in Chaitamug. Maximum number of branches per plant was obtained in the BARI mung-7 and minimum was obtained in BU mug1. The highest and the lowest total chlorophyll content were recorded in BARI mung-7 and Chaitamug respectively. The highest number of pods per plant was recorded in BARI mung-8 and the lowest was recorded in Sonamug. Maximum pod length was observed in BARI mung-7 and minimum length was observed in Chaitamug. In terms of number of seed per pod, the highest and lowest results were found in BARI mung-8 and Chaitamug respectively. Pod sterility percentage was maximum in Chaitamug and minimum in BARI mung-7. The highest weight of 1000-seed was recorded in BARI mung-6 and the lowest was recorded in BARI mung-8.The highest yield per plot was obtained from BARI mung-7 and the lowest yield per plot was obtained from Chaitamug. From the study, it was observed that minimum disease incidence and severity were appeared on BARI mung-7 in natural field condition and gave the best performances in respect of yield and yield contributing characters.