IN VITRO REGENERATION OF Caladium bicolor

Abstract

The experiment was conducted at the Biotechnology Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period from October 2013 to July 2014 to investigate the effects of BA, Kin, IBA, IAA, NAA for in vitro regeneration of Caladium bicolor using shoot tip explants. The work was designed in CRD with three replications. Maximum number of shoot (5) initiated when MS media was supplemented with 1.0 mg/L BA and minimum (1) was at control. Highest percentage (90%) of explants showed shoot induction at BA 0.50 mg/L +2.00 mg/L IBA and minimum shoot induction days (40.47) were recorded at BA 1.00 mg/L +2.00 mg/L IBA. Highest number of shoots (19.67) were recorded at BA 1.00 mg/L +2.00 mg/L IBA. Longest length of shoot (11.43 cm) was recorded at BA 0.25 mg/L +2.50 mg/L IBA. Maximum number of leaves were recorded at BA 1.00 mg/L +2.00 mg/L IBA. To investigate root formation potentiality and root morphology NAA, IAA and IBA at different concentrations were used. Among the rooting hormones IAA at 2.0 mg/L concentration showed maximum percentage (90%) of root induction. Maximum numbers (29.87) of root were recorded when IAA was used at 2.0 mg/L concentration. Highest length of root (10.20 cm) was recorded at 2.0 mg/L IAA. Ultimately combined effect of BA and IBA seemed better than individuals based on average performance of growth parameters. Survival rate was 92% in open field condition. Reliable protocols for micropropagation of Caladium bicolor were established and could be used for large scale production of disease free, high yielding and premium quality planting material.