STUDY ON POTATO (Solanum tuberosum L.) REGENERATION CAPABILITY IN AMMONIUM NITRATE FREE MEDIUM COMPOSITION

Abstract

The experiment was conducted at the Biotechnology Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period of January, 2016 to June, 2017 to evaluate the performance of ammonium nitrate free medium for in vitro regeneration of a potato (Solanum tuberosum L.) variety, Asterix. Sprouts and nodal segments were used as explants. A new chemical which is indicated as β chemical had been used in this research. Five different compositions of the stock solution-01 of the MS medium were used which were marked as Treatment-01 (standard MS dose, 1962), Treatment-02 (NH 4 free stock solution), Treatment-03 (NH 4 NO 3 free stock solution with double dose of other ingredients), Treatment-04 (stock solution-01, developed in the Department of Biotechnology, SAU, Dhaka ) and Treatment-05 (readymade MS powder). In vitro regeneration of a potato was successfully achieved in those media. The parameters like shoot length, shoot number, shoot diameter, node number per plantlet, leaf number per plantlet were highest in Treatment-04 at 14, 21 and 28 days of regeneration. But in case of root number and root length (cm) per plantlet, Treatment-05 showed the highest result and in some cases it was statistically similar with Treatment-04. Treatment-02 showed lowest result in each of the said parameter. It might be due to the low concentration of nitrogen and it indicated that NH 4 x

NO 3 is essential for in vitro regeneration of potato. Treatment-03 performed moderately in most of the parameters. Asterix variety showed significant variation in case of Treatment-01

and Treatment-05

on most of the parameters under studied. Therefore, a relliable medium composition for in vitro potato regeneration has been established. So, the stock solution-01 which was developed from the Department of Biotechnology, SAU, Dhaka can be the best alternative to NH 4 in tissue culture study of plants.