Abstract:
The
experiment was conducted . at the Proshika plant tissue culture
Laboratory,Manikganj,Dhaka during the month of September 2007 to May 2008 to
study Cloning of ginger through
in vitro
Techniques. Sprouted buds from rhizome of
ginger (variety Thai) were cultured in four different basal medium with diifercnt
hormone (NAA, IAA, BA, KN, RAP, IBA & ADS) and their combination to lind out
suitable combination of media and also find out the best soil type for its successive
growth and development. In case of single hormone NAA at (1mg/I) concentration
with MS media showed highest shoot length (3.0cm), highest leaf number (2.2) and
multiple tillers (2.2). In combined effect BA (2mg/I) and KN (1mg/I) with MS medium
showed the best performance, with highest pseudostem length (1.4cm), liigicst leaf
numbcr(2.4) and highest multiple tiller (2.0). In case of LS medium with organic
supplement and hormone RAP with KN with tomato juice gave highest shoot length
(5.0cm) with 78% of rooting. In case of rooting hormone IBA at (1mg/I) with MS and
White media achieved 82% root. For soil, garden soil: sand: soil (I 1:1) showed
highest growth rate (plant height 11 .Scni),It may he concluded that shoot induction
was good in MS media and root induction was best in MS and \Vhite liquid rooting
media.
