GENERATING DOUBLED HAPLOID MUSTARD (Brasska rapa L.) PLANTS THROUGH ANTHER CULTURE

Abstract

The present study was undertaken in the Tissue Culture Laboratory of the Department of Biotechnology. Sher-e-Bangla Agricultural University. Shcr-e-Bangla Nagar, Dhaka- 1207, during the period of August 2010 to March 2012 generated calli. shooting through anther cultuc for doubled haploid ci' mustard (Brassica rapa L.) plants. There were three genotypes ofF1 generation of SAU Sharisha IX Tori 7. BARI Sharisha 15 X Tori 7 and BARI Sharisha 6 X Tori 7 and nine hormonal combination treatments were used 11w callus development and fifteen hormonal combination treatments were used for shooting under present study. Anthers were used as explants. Six different diameter of [lower buds (1.00-1.50. 1.51-2.00. 2.01-2.50. 2.51-3.001, 3.01-3.50 and 3.51-4.00 mm) and six different cold pretreatment (40c) (2, 3. 4. 5. 6 and 7 days) and their combined effect was studied for callus induction. From experiment I it showed that highest percentage ( 70%) when 3.01-3.50 mm flower bud size are used in combination with 4 days cold pretreatment (4°(') in the genotype ofF1 generation of SAU Sharisha Ix Tori 7. From experiment 2 it showed that the minimum (4.2006 days) days to callus induction at T5 4.0 mg/f. 2. 4-D 4.0 mg/L BAP) in genotype of F 1 generation SAU Sharisha I x Tori7. The maximum breadth (1.520 cm at 35 DAI), length (1.920 em at 35 DAI) and size (1.720 cm at 35 DAI) was observed in combination of T7(6.0 mg/L 2. 4-D2.0 mg/L BAP) and the genotype F1 generation SAU Sharisha I x Tori7. The maximum (0.7020 and 1.302 gm at 14 and 35 DAI respectively) callus weight was observed in combination l'7(6.0 mg/L 2, 4-D±2.0 nigfL BAP) and the genotype oiF1 generation of BARI Sharisha 6 x Tori 7. The minimum days to shoot initiation (4.20days) was observed in the genotype ofF1 of BARI Sharisha 15 x Tori 7 with T12 (1.0 BAP + 0.5 2,4-D).