CALLUS INDUCTION AND IN VITRO PLANT REGENERATION OF BARI RELEASED POPULAR POTATO (Solanum tuberosuin L.) CULTIVARS

Abstract

The present study was undertaken in the Genetics and Plant Breeding Laboratory and the 'lissue culture laboratory of the Department of Biotechnology. Sher-e-Baiigla Agricultural University, Sher-e-Bangla Nagar. Dhaka- 1207. during the period of January 2011 to December 2011. to investigate the in vitro perkrmance ot three Bangladesh Agricultural Research Institute ( BARI) released popular potato eultivars viz. Granola. Diamant and Asterix using potato sprout as expLant. live Levels of' (A (50. 100. 200. 300 and 400 ppti/l.) were used to find out the best concentration for sprout initiation, and it was revealed that (ib\ at the rate of 400 ppm/I. was the best concentration for maximum sprouting within short period of time. The effect of ditThrent combination and concentration of NAA and BAP on in vitro callus induction and plant regeneration was also studied. the concentrations for NAA were 1.0 mg/I. and 1.5 mg/!. and fOr BAP were 0.5 mg!L .. 1.0 mg/L. 1.5 mg/I. and 2.0 mg/L. The combined ctThet of both the honnones were also studied. the variety Diamant produced callus in the shortest period of time (4.46 days). It took minimum days 3.53 days) for callus induction in the treatment of' LU mulL NAA f 1.5 mg/I. BAP. The largest size (2.10 cm) and the highest weight (2.54 gm) of callus were noticed in the variety Diainant with the treatment 1.5 mg/L NAA within 28 DA I. In ease of shoot initiation, variety Granola took minimum time (13.17 days) followed by Diamant (1 3.63 days) and Asterix (15.01) days). The variety Granola produced the maximum number of' shoots'plantlet (3.00 shoots/plantlet) and the Longest (6.76 cm) shooise'plantlet ibllowed 1w Diamant (2.12 shoots/plantlet and 6.51 cm) and Asterix (1.91 shoots.'plantlet and 5.68 cm). respectively. Variety Granola also produced the maximum number of leaves/plantlet (20.0 leaves) with the treatment 1.5 mg/I.. NAA+l .5 mg/I. BAP within 28 DAI. The variet Asterix took the minimum (12.73 days) days to root initiation followed by Granola (15.01 days) and Diamant (15.48 days). In treatment 1.5 mg/I. NAA-0.5 mg/I. BAP required the minimum (0.93 das) days to root initiation and also the number of roots/plantlet (1 4.53 roots/planilet at 28 DAI ) was the maximum. When variety and treatment took tinder consideration, the treatment 1.5 mg/L NAA±0.5 mg/L. HAl' with variety Dianiani produced the maximum (19.40 roots/plantlet) and Granola produced the longest 14.42 cm) roots/plantlet respectively. The regenerated plantlets were transIrred successfully in plastic tray and subsequently to soil. The variety Graiola survived at high rate in growth chamber and in open atniosphere. Among all the varieties. Granola showed the best pertonnanee from callus induction, plant regeneration to in vivo establishment.