Abstract:
The experiment was undertaken with a view to standardize a protocol for in
v/tm culture and plant regeneration using potato meristem as explants. The
experimental materials were six potato genotypes viz, Shil Bilati, Kufri
Shinduri, Lal Pakri, Shil Pakri, Jam Alu and Pahari Pakri. Four levels of GA3
(100, 200, 300 and 400 ppm) were used to assess the influence of GA3 on
sprouting abilities of six potato genotypes. The maximum sprouting efficiency
was observed in 400ppm 0A3 treatment. Combinations of GA3, 2, 4-D and
Kinetin were used along with fresh MS media to inoculate meristems and to
induce callus. Maximum size of callus was observed (0.82 cm) in Kufri
Shinduri inoculated in T4 (0.5 mg/I GA3 +1.0 mg/I 2, 4-0+ 1.5 mg/I KIN). Shil
Pakri meristem inoculated in hormonal treatment T3 (0.5mg/i GA3 + 0.5mg/I
2, 4-D + 1.5mg/I KIN) showed best results regarding highest single shoot
length/plantlet (5.88 cm). Three levels of GA3 treatments (fresh MS,
MS+0.5mg/l GA3 and MS+1.5mg/1) were used to study subsequent shoot
elongation and plant regeneration of single shoot/plantlet produced from
meristem culture. Treatment T2 (0.5 mg/I GA3) gave maximum plant height
(5.68 cm), number of branches/plantlet (2.60), root lengthfplantlet (8.74 cm)
and number of leaves/plantlet (14.86). Shil Pakri gave maximum performance
in respect of plant height (8.96 cm), number of branches/plantlet (2.78),
number of roots/plantlet (4.33) and number of leaves/plantlet (22.56). Shil
Pakri treated with T2 (0.5 mg/I GA3) showed maximum plant height (9.50 cm),
number of branches/plantlet (5) and number of leaves/plantlet (33.67). Among
all genotypes Shil Bilati was least responsive whereas Shil Pakri showed
better overall performance from meristem culture to establishment of plants in
field conditions. Plantlets obtained from meristem culture were tested using
DAS-ELISA protocol for confirming plantlets to be virus free. All the potato
genotypes except Shil Bilati and Jam Alu were reported virus free.
xiv
Description:
A Thesis
Submitted to the Faculty of Agriculture.
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements for the degree of
MASTERS OF SCIENCE
IN
GENETICS AND PLANT BREEDING
SEMESTER: JULY- DECEMBER, 2010
