PLANT REGENERATION AND TRANSFORMATION IN KENAF (Hibiscus cannabinus)

Abstract

Two different sets of experiments were conducted at the l3iotechnology Laboratory. Department of Genetic Resources and Seed Division. Bangladesh Jute Research Institute, Dhaka during the period of January 2007 to November 2007. In the first experiment, a detailed investigation was carried out to study the callus induction ability and subsequent plant regeneration protocol for two varieties of kenaf (I IC-2 and 1 C-95) using cotyledon with attached petioles as explants. In the second experiment. investigation was carried to study Açgiohc:ci rirun mediate genetic transformation. Two different investigations were made in the liNt experiment to slioss the effect of diflerent age of explants and BAP concentration in plant regenerati okenn oaff.. Five diliërcnt ages (6. 8. 10. 12 and 14 days) explants and seven different BAP concentrations were used and wide ranges of variation were observed. I lighest callus production (91.7%) and shoot regeneration (85.3%) 'cre found in I IC-2 when 10 days old explains were used. l3oth 1IC:-2 and I l('-95 showed the maximum callus initiation (91.7%) and the highest shoot regeneration (91.7%) in I lC-2 when MS medium supplemented with 3.0 mg/I BAP and 0.5 mg/I IAA was used. .1gmbacteriznn lumefuewis strain L8A440-1 was used Ibr transformation. containing selectable marker gene npIIl conferring resistance to kanamycin and GUS reporter gene also. Between the varieties. I IC-2 showed the highest response to GI.S expression (90.0% positive). Transgenic shoots produced roots and transferred to the soil successililly.