Abstract:
The experiment was conducted in the Disease Diagnostic Laboratory and Net
house of Department of Plant Pathology, Sher-e-Bangla Agricultural
University, Dhaka during the period of July 2012-June 2013 to find out suitable
antagonistic bacteria against Ralstonia solanacearum causing bacterial wilt of
tomato. Fourteen probable antagonistic bacterial isolates were isolated from
rhizosphere soil of tomato and 6 isolates of Rhizobium leguminosorum
collected from the department. In-vitro screening of these bacteria were done
against R. solanacearum to detect the promising antagonistic bacteria.
Ralstonia solanacearum was isolated from the wilt infected part of tomato
plant and it’s morphological, biochemical and cultural features were studied.
The bacterium was gram negative, rod shaped and showed positive results in
KOH solubility test, starch hydrolysis test, catalase test, oxidase test, citrate
utilization test, motility indole urease agar
0
C on TTC medium. Out of all antagonistic bacterial
isolates, Pseudomonas fluorescens ...
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University,
Dhaka, in partial fulfillment of the requirements for the degree
of
MASTER OF SCIENCE
IN
PLANT PATHOLOGY
SEMESTER: JULY- DECEMBER, 2013