Abstract:
Two sets of' (lie experi nents were conducted at the Genetic Engineering Laboratory,
Genetic Resources and Seed Division, Bangladesh Jute Research institute (13J1U).
Dhaka, during the period of' July 2006 to June 2007, to fulfi I the objectives of the
study. In the first set of the experiments, two varieties of C. o/itorius were used to
investigate their in vitro regeneration potentiality, and in the second set of the
experiments, two varieties of C. olilorius were used to observe their transformation
ability. Cotyledons (with attached petiole) were used as explants. The percentage of
germinated seeds (90.00%) on cotton-supported medium found to be much higher
than germinated seals (68.33%) on agar-supported medium. The seedlings grown on
cotton-supported medium found to be much more healthier than seedlings grown on
agar-supported medium. Maximum no. of plant regeneration percent was obtained
From the eutyledonary petioles of the varieties of C. olitorws on MS agar solidified
medium supplemented with 0.5 ing/l IAA and 3.0 mg/I BAP. Plant regeneration was
also observed at different concentrations oflTeSO4 and it was found that. 56 mg/I gave
the highest result. The efficiency of plant regeneration froni the cotton-supported
seedlings was better than the agar-supported seedlings. The plantlets produced roots
on hormone free MS medium rapidly. The effects of non—ionic surfaetants (Pluronie
F-OS) on shoot regeneration for jute (C. olitorius) coty edons was assessed.
Supplementation of culture me(' in with 0.01 -0.08% (w'v) Pluri. nic F-OS increased the
mean percentage of cotyledon producing shoots, in the second set of the eXperil)lentS,
an efficient and reproducible protocol for the production of transgenic jute plant was
developed by inoculating cotyledonary petioles with A. tu,aefciciens strain LBA4404
carrying a binary vector pBl 121, which contains selccable marker gene nptll
conferring resistance to kanamycin and the GUS reporter gene. After co-cultivation
and selection. histochemical GUS assay was performed in two varieties (0-9897 and
0-72). In the transformed explants, GUS reporter gene was expressed showing blue
colour in the explant tissues. Non-transformed explants did not show any colour.
I3etwcen the varieties, 0-9897 showed the highest response to GUS assay (60.00%).
Description:
A Thesis Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University,Dhaka,
in Partial Fulfillment of the Requirements
for the Degree of
Master of Science in
Genetics and Plant Breeding
Semester: January- June, 2007
