Abstract:
An experiment was conducted during the period of July 2006 to May
2007 in the Genetic Engineering Laboratory, Genetic Resources and Seed
Division, Bangladesh Jute Research Institute, Dhaka, Bangladesh.. Kcnaf
variety I-IC-2 was used to investigate the in vi'ro regeneration
potentiality. Different concentrations and combinations of hormones,
vitamins, FeSO.1 and surfactant (F-68) were supplemented to MS medium
to observe callus induction and regeneration ability of kenaf. In cottonsupported
MS liquid medium, the germination percentage was higher
(91.00%) compared to agar supported MS medium (82.34%). Eight days
old seedlings were highly responsive to shoot regeneration. Among the
phylohormonc combination BA!' (3.0 mg/I) and IAA(0.5 mg/I) with MS
supplemented showed the highest shoot regeneration. In different
concentrations of vitamin, the highest percentage of shoot regeneration
was obtained (81.25%) at 4.0 mg/I vitamin concentration. Among
different concentrations of surfactant, the highest percentage was
observed (81.00%) in 0.5% surfactant concentration. Using different
concentration of' FeSO4 , the highest percentage of shoot regeneration was
(9 1.67%) at 2.0 mg/I PeSO4 concentration. In present study, an efficient
protocol for the plant regeneration has been developed which could be
further used for genestic transformation in kenaf.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Shcr-c-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
GENETICS AND PLANT BREEDING
SEMES'I'ER JANUARY-JUNE 2007
