Abstract:
An experiment was conducted at the Plant Pathology laboratory and Mushroom Culture
House (MCH) in the Department of Plant pathology, Sher-e-Bangla Agricultural University
(SAU), during October, 2021 to April 2022, to investigate the prevalence of microorganisms
on mushroom substrates and the efficiency of chemicals and botanicals on contaminations,
growth and yield of maple oyster mushroom. The experiment was consisted of single factor
and followed Completely Randomized Design (CRD). Sugarcane bagasse was treated with
different botanicals and fungicides viz: Sulcox 50 WP (Copper oxychloride), Indofil M45
(Mancozeb), Mancer 75WP (12% Carbendazim+ 63% Mancozeb), 3% Hydrogen Peroxide,
Lanatana (Lantana camera) and Nimbicidine with autoclaving. Different application along
with sugarcane bagasse showed significant variations in respect of all characters of
mushroom. Severity of contamination was varied from 4-66%, while during the 3
harvest,
66%, 4% and 12% contamination was observed in control, Sulcox-50WP and Indofil M-45,
respectively. No severity of contamination was found when the substrate was supplemented
with botanicals after autoclaving. The highest growth rate (0.45 cm/day), the maximum
number of primordia /packet (82.4), the maximum number of effective fruiting bodies
/packet ((42.2), biological yield (157.04 g), economic yield (125.0 g) were recorded from
Copper oxychloride treated packet, while the lowest performance was obtained from control
in all cases. Trichoderma harzianum and Aspergillus niger were isolated and identified from
contaminated spawns. Under In-vitro evaluation of chemicals, maximum inhibition of
Trichoderma harzianum (68.76%) and Aspergillus niger (91.28%) were recorded from 3%
hydrogen peroxide treated plate. In case of botanicals, Aspergillus niger (41.96%) and
Trichoderma (30.67%) were inhibited by Lantana, while, Trichoderma (27.7%) and
Aspergillus niger (26.16%) was inhibited by Nimbicidin. The economic yield of oyster
mushroom was correlated positively with number of fruiting bodies and the biological
efficiency. It may be revealed that supplementation with botanicals after autoclaving is the
best option to mitigate the substrate contamination of maple oyster mushroom.
