INFLUENCE OF DIFFERENT FACTORS ON PLANT REGENERATION SYSTEM OF JUTE SPECIES

Abstract

Experiments were conducted during the period from August 2005 to May 2006 in the Genetic Engineering Laboratory. Depailment of Cytogenctics, Bangladesh Jute Research Institute (IIJRI), i)haka. In the first experiment, five varieties of ( 'orc/wntc sp were used to investigate their in ti/ru germination percentage. And in the second experiment, five varieties of ('urc/wnts sp. were used to investigate their in ri/tv regeneration performance. In the third experiment, varieties of ('vivijoris's sp were used to investigate their in ri/ru shoot regeneration from the explants (cotyledons) of C. capsularis and C. ui/forks's with different hormonal concentrations. Fourth to ninth experiments were conducted to show the different factors on plant regeneration from cotyledons of C. capsularis and C. v/i/onus. In the first experiment, seed germination percentage was found highest in CVE-3 in cotton-based medium (98.88%) compared to agar-based medium (87.17%). Among the phytohormone combination, MSi 2 rug/I BAt' I 0.5 mg/I IAA showed the highest shoot regeneration (60.66%). Among the varieties, CVE-3 was highly responsive to shoot regeneration (67.99%). MS media without honnonc (MSO) was used for root formation. The variety CVE-3 response better than 0-72 towards shoot regeneration at different pt-I level. Among the sucrose concentration 3% (30mg/I) showed the highest shoot regeneration (87.49%). Among the different concentration of vitamins x2 times (3.0mg/I) showed the highest shoot regeneration. Using ditThrent concentration of surfactant 0.1% surfactant showed the highest shoot regeneration (91.663%). In the NaCl experiment, among the three varieties CVE-3 was found highly responsive to shoot regeneration (97.22%) and 0.125, 0.25 and 0.50 (%) shows the better responsive to shoot regeneration. Using different concentration of FeSO4, x2 times (3.0mg/I) showed the highest shoot regeneration. The variety CVE-3 response better than 0-72 towards shoot regeneration at different concentration of FcSO4,