Abstract:
Experiments were conducted during the period from August 2005 to May 2006 in the
Genetic Engineering Laboratory. Depailment of Cytogenctics, Bangladesh Jute Research
Institute (IIJRI), i)haka. In the first experiment, five varieties of ( 'orc/wntc sp were used
to investigate their in ti/ru germination percentage. And in the second experiment, five
varieties of ('urc/wnts sp. were used to investigate their in ri/tv regeneration
performance. In the third experiment, varieties of ('vivijoris's sp were used to investigate
their in ri/ru shoot regeneration from the explants (cotyledons) of C. capsularis and C.
ui/forks's with different hormonal concentrations. Fourth to ninth experiments were
conducted to show the different factors on plant regeneration from cotyledons of C.
capsularis and C. v/i/onus. In the first experiment, seed germination percentage was
found highest in CVE-3 in cotton-based medium (98.88%) compared to agar-based
medium (87.17%). Among the phytohormone combination, MSi 2 rug/I BAt' I 0.5 mg/I
IAA showed the highest shoot regeneration (60.66%). Among the varieties, CVE-3 was
highly responsive to shoot regeneration (67.99%). MS media without honnonc (MSO)
was used for root formation. The variety CVE-3 response better than 0-72 towards shoot
regeneration at different pt-I level. Among the sucrose concentration 3% (30mg/I) showed
the highest shoot regeneration (87.49%). Among the different concentration of vitamins
x2 times (3.0mg/I) showed the highest shoot regeneration. Using ditThrent concentration
of surfactant 0.1% surfactant showed the highest shoot regeneration (91.663%). In the
NaCl experiment, among the three varieties CVE-3 was found highly responsive to shoot
regeneration (97.22%) and 0.125, 0.25 and 0.50 (%) shows the better responsive to shoot
regeneration. Using different concentration of FeSO4, x2 times (3.0mg/I) showed the
highest shoot regeneration. The variety CVE-3 response better than 0-72 towards shoot
regeneration at different concentration of FcSO4,
Description:
A Thesis
Submitted to the Faculty of Agriculture.
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
GENETICS AND PLANT BREEDING
SEM ESTER: JAN tIARY-.J UN E, 2006
