Abstract:
An experiment was conducted in the Genetic Engineering Laboratory of the
Cvtogeneties Department, Bangladesh Jute Research Institute (BJRI), Dhaka
during the period from Januan to July 2006. Cotyledons (with attached petioles
of mesta (Hibiscus sabdar?fth L.) were used as explant to investigate their in vitro
re2eneration potentiality. Seed germination percentage was found highest on
clinical cotton supported MS liquid medium (901/-0') than agar-based MS solidified
medium (81.33%). Amone the combination. MS medium + SAP (2.0 m1) + IAA
(0.5 mg/I) showed the highest shoot regeneration (58.33). No shoot regeneration
was observed in MS media without hormone (NISO). Maximum shoot
multiptication (46.67%) •as thund from shoot apex on XIS medium - BAP (2.0
m.1) - IAA (0.5 nw/I). MS medium with hormone and MS medium nithout
hormone (MSO) were used for root formation from shoots. The highest number of
initial roots per shoot (6.671 as found in the presence of 1AA (2.0 mg 1. [he
maximum weight of roots per shoot (0.21 20 was obtained in IAA (1.0 mg 1. The
highest number of' days to root initiation (9.00) was required in. IAA (0.5 mgb
and the lowest (5.00) in IAA (0.0 mg'l) containing MS medium. The regenerated
plantlets of mesta were successfully in soil.
Description:
A Thesis
Submitted to the Faculty of Agriculture
Shcr-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
GENETICS AND PLANT BREEDING
SEMESTER: JAN UARY-JLNE, 2006
