Abstract:
Mung bean (Vigna radiata (L.) R. Wilczek) has been widely used as a pulse crop. The
study was carried out at the Biotechnology laboratory of Sher-e-Bangla Agricultural
University, during the period of July 2021 to June 2022 to investigate molecular diversity
and fingerprinting of fifteen mung bean germplasm. RAPD primers were selected and
synthesized for molecular diversity analysis. All of the primers were able to produce
polymorphic bands. The highest number of bands were observed in RAPD primer OPT20.
The polymorphic Information Content (PIC) of RAPD markers ranged from 0.24 to
0.32 with an average value of PIC =0.27. Gene diversity ranges from 0.41 to 0.28 with an
average value of 0.33. Primer showed OPBC-1, OPA-20, OPP-03, OPT-20 moderate
diversity and primer OPBC 18 showed low diversity on the basis of PIC value. The value
of pair -wise comparisons of Nei’s (1983) genetic distance (D) among 15 genotypes was
computed from and it was ranged from 0.1034 to 0.6207 with an average of 0.3621.
Dendrogram based on Nei’s genetic distance using unweighted Pair Group Method Of
Arithmetic Mean (UPGMA) indicated the segregation of 15 genotypes into four main
clusters. This result can be conveniently used for molecular diversity analysis to identify
diverse parent for mung bean improvement program.
Description:
A Thesis
Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
For the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
