IDENTIFICATION OF MULTIDRUG-RESISTANT ESCHERICHIA COLI AND SALMONELLA SPECIES ISOLATED FROM CHICKEN AND PIGEON IN SOME SELECTED AREAS OF DHAKA CITY

Abstract

The poultry industry in Bangladesh is considered an important sub-sector for economic growth and employment. Chicken and pigeon both are raised in the farm and sold in the live bird markets. This activity, therefore, may pose potential threats for humans as well as other animals as poultry may carry and spread different pathogens including drug- resistant bacteria. This work concentrated on the isolation and identification of multi-drug resistance E. coli and Salmonella spp. from chicken and pigeon.Forty five cloacal samples were collected from 45 birds (chicken and pigeon) during the study. E. coli and Salmonella spp. were isolated and identified on various types of agars. Biochemical tests were also performed. Besides, the presence of E. coli and Salmonella spp. were further confirmed by polymerase chain reaction (PCR). Moreover, antimicrobial susceptibility testing of the isolates was performed against eleven antibiotics from seven classes on the Kirby–Bauer disk diffusion method. The overall prevalence of E. coli and Salmonella spp. was 84.45 and 53.33%, respectively. The antibiogram profile of 38 E. coli as well as 24 Salmonella spp. revealed that all isolates were resistant to more than antibiotics. Hundred percent (100%) of E. coli showed resistance against amoxicillin, ampicillin and erythromycin. Furthermore, E. coli found sensitive against ceftriaxone and gentamicin. Similarly, around 100% of the Salmonella spp. showed resistance against amoxicillin, ampicillin and Co-Trimoxazole, respectively. However, 66.67% and 58.33% Salmonella spp. were found to show sensitivity against gentamicin and ceftriaxone. However, all isolated E. coli and Salmonella spp. were Multidrug-resistant (MDR). Poultry species carrying MDR E. coli and Salmonella spp. may contribute to the transmission and spread of these microorganisms. Therefore, strict hygienic measures should be taken during the farming & selling to decrease the potential transmission of E. coli and Salmonella spp. from chicken and pigeon to humans as well as other animals.