Abstract:
The experiment was carried out during the period from November 2020 to March 2021
to find out the effect of substrate of mother spawn on contamination and productivity
of oyster mushroom (Pleurotus ostreatus). Nine different treatments were used as viz.
T
1
= Mustard; T
2
= Red maize; T
3
= 50% rice straw and 50% sawdust; T
4
= Millet; T
6
= Gram; T
7
= Pea; T
8
= Rice and T
9
= White maize. The experiment was
laid out in single factor Completely Randomized Design (CRD) with replications. The
highest mycelium running rate of mother spawn (0.85 cm/day) was observed when the
rice grain was used. The minimum duration required from incubation to completion of
mycelium running of mother spawn (16.33 days) and maximum mycelium weight
(4.08) was observed in rice. The minimum duration required from incubation to
completion of mycelium running in yield packets (18.33), from stimulation to
primordial initiation (5.89 days), from primordial initiation to 1st harvest (5.67 days)
and the lowest total harvesting period (40.00 days) were found when mother spawn of
rice was used. Again, the maximum number of primordia (87.33), effective fruiting
body per packet (31.00), the highest length of pileus (5.17 cm) and width of pileus (7.49
cm) were found at mother spawn of rice. Oyster mushroom grown on sawdust
substrates where mother spawn of rice inoculated gave the highest biological yield
(175.67 g), economic yield (168.33 g) and biological efficiency (35.13%). During the
cultivation period four contaminants namely Trichoderma sp. Penicillium sp, Rhizopus
stolonifer and Aspergillus niger were isolated and identified from contaminated
substrates. Percent contamination of fungi gradually increased with the increase of days
after stimulation. After 45 days after stimulation (DAT), packets inoculated with rice
and wheat mother spawn were not contaminated. Among the treatments, the
performance of mother spawn of rice was better economically as well as growth and
yield performance for the cultivation of oyster mushroom.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
In partial fulfillment of the requirements for the degree
of
MASTER OF SCIENCE
IN
PLANT PATHOLOGY
