Abstract:
An experiment was conducted in Dr. M. A Wazed Miah Central laboratory, Department
of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University,
Sher-e-Bangla Nagar, Dhaka-1207 during the period from June’ 2020 to December’
2020 to detect, identify and isolating associated mycoflora in three varieties of
sunflower for fullfilling the objectives with five methods viz., Blotter paper pethod,
Deep freezing method, Washing test, PDA media method and Water agar plate method
were used. The experiment was laid out in Completely Randomized Design (CRD) with
three replications for all methods. The species composition, percentage of seed infection
and seeds germination percentage differed among cultivars as well as method. A total
of 9 different fungal species belonging to six genera were detected from sunflower seed
through five different methods. Among them BARI Sunflower 3 showed the highest
infection in washing test compared to other methods. A number of fungi isolated in the
present study specially those in the genera viz. Alternaria sp, Curvularia sp, Aspergillus
sp, Fusarium sp, Rhizopus sp, and Stemphyllum sp are known to be potent mycoflora
producers. Among the fungi detected, frequency of Alternaria alternata was found
significantly the highest in the all methods. The highest percentage of germination was
found in BARI Sunflower 3 (86.67%) by Blotter paper method. The highest percentage
of infection was found in BARI Sunflower 3 (73.33%) in both Blotter and Washing test
and the percentage of dead seed after incubation was least in BARI Sunflower 3
(13.33%) by Blotter paper method. Of the five methods tested for detection of seedborne
mycoflora, Blotter paper, PDA media, and Washing test were highly effective for
mycoflora study.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
In partial fulfillment of the requirements for the degree
of
MASTER OF SCIENCE
IN
PLANT PATHOLOGY
