Abstract:
The present study was conducted to determine the occurrence of aflatoxin residues in
rice, wheat, maize, lentil, mungbean, mustard, soybean, peanut and processed food
items and to identify the sources of contamination with risk assessment. To perform
this experiment, 29 samples were collected from godown, 21 samples were collected
from wholesale market, 10 processed food samples were collected from departmental
store. So, total 60 samples were collected from different locations of Bangladesh. The
study duration was March 2020 to June 2021. Collected grain samples and processed
food samples were analyzed using High Performance Liquid Chromatography (HPLC)
with fluorescence detector to detect residues of aflatoxin (B
1
, B
2
, G
1
). In this
study, 8 godown samples (3 peanut samples, 2 maize samples, 3 lentil samples) and 1
wholesale market sample (rice) were detected with aflatoxin, whereas no processed
food sample was detected positive for aflatoxin. The highest concentration of aflatoxin
found in peanut (local) was 156.12 µg/kg and lowest concentration found in peanut
(imported) was 0.5181 µg/kg. Results showed that among 9 samples, 4 samples (1
peanut sample, 2 maize samples and 1 lentil sample) exceeded the maximum residue
limit (MRL) of aflatoxin B
1
(2 µg/kg). Among 4 samples, 3 samples (1 peanut sample,
2 maize samples) exceeded the maximum residue limit (MRL) of total aflatoxin B
1
,
G
1
& G
2
(4 µg/kg) as per Commission Regulation (EC) No. 1881/2006 for food. Human
health risk assessment from aflatoxins exposure through rice, maize, lentil and peanut
consumption from the godown and wholesale markets by adults showed no significant
adverse health risk to humans.
Description:
A Thesis
Submitted to the Department of Agricultural Chemistry
Sher-e-Bangla Agricultural University, Dhaka-1207
In partial fulfillment of the requirements for the degree of
MASTER OF SCIENCE
IN
AGRICULTURAL CHEMISTRY