ISOLATION, IDENTIFICATION AND ANTIBIOTIC SENSITIVITY PROFILING OF ESCHERICHIA COLI AND SALMONELLA SPP.: AN ISSUE OF PUBLIC HEALTH

Abstract

The study's goal was to look at the isolation and identification of Escherichia coli and Salmonella spp. bacteria and their antibiotic resistance patterns. A total of 40 samples of duck liver and intestinal contents was collected and selected from different village on Savar upazila and tested for E. coli and Salmonella spp. bacteria with the isolates subjected to antibiotic sensitivity tests. Cultural characteristics, biochemical testing, and gram's staining were used to isolate and identify bacterial genera/species. The prevalence rates are different for different bacteria. Highest prevalence rate found in E. coli (52.5%) followed by Salmonella spp. (37.5%). Antibiotic sensitivity test by disc diffusion method or Kirby-Bauer test was performed against five most used different antibiotics. Ciprofloxacin was the most sensitive to E. coli isolates (95.24 %), followed by gentamycin (66.67%), while amoxicillin (90.47%) followed by tetracycline & amoxicillin both (80.95%) was the most resistant. Salmonella spp. isolates were found to be the most susceptible to Ciprofloxacin (100%), followed by Gentamycin (86.67%). Highest resistant pattern of Salmonella spp. was showed against amoxicillin (100%) followed by ampicillin (80%) and tetracycline (60%) with intermediate resistant 40%. According to the findings of this study, duck contains multidrug resistant E. coli and Salmonella spp. pathogens on both duck liver and intestinal contents. Salmonella spp. and multidrug-resistant E. coli are dangerous bacteria that can spread to people by contact with them or through the food chain, raising major public health problems.