Abstract:
This study was carried out in Sher-e-Bangla Agricultural University, Dhaka
for isolation, identification and antibiotic sensitivity profiling of Escherichia
coli and Salmonella spp. from dead broiler in local market of Dhaka city. A
total of 150 liver and heart samples were collected from dead broiler. The
microorganisms of Escherichia coli and Salmonella spp. were isolated and
identified. Samples were collected aseptically and inoculated onto various
culture media for isolation of Escherichia coli and Salmonella spp.
Identification of bacteria from positive samples was performed by cultural
characteristics and biochemical tests. Hundred percent prevalence with
microorganism was found from all the samples. The prevalence of E. coli
and Salmonella spp. in liver samples was (45.33%) and (40%) and in heart
samples it was (44%) and (38.67%) respectively. The overall prevalence of
E. coli and Salmonella spp. in broiler was (44.67%) and (39.33%).
Antibiotic sensitivity profiling of the isolated E. coli and Salmonella spp.
was performed by the disc diffusion method against 10 commonly used
antibiotics. The highest rate of sensitivity against E. coli was found with
Ceftriaxone (52.24%) followed by Gentamicin (49.25%) and Streptomycin
(44.78%). The highest rate of resistance was recorded in Tetracycline
(52.24%) followed by Ampicillin (50.75%), Cefuroxime (47.76%),
Amoxycillin (46.27%) and Co-Trimoxazole (46.27%). The highest rate of
antibiotic sensitivity against Salmonella spp. was found with Ceftriaxone
(52.54%) followed by Gentamicin (50.85%) and Streptomycin (47.46%).
The highest rate of resistance of Salmonella was recorded in Cefuroxime
(54.24%) followed by Ampicillin (52.54%), Cefixime (50.85%),
Tetracycline (49.15%), Amoxycillin (47.46%) and Co-Trimoxazole
(45.76%). Based on the present study, it may be concluded that Ceftriaxone
should be best result against both E. coli and Salmonella spp. followed by
Gentamicin and Streptomycin under the present study.
Description:
A Thesis
Submitted to the Department of Medicine & Public Health
Sher-e-Bangla Agricultural University, Dhaka
In partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
MEDICINE
