SUPPLEMENTATION OF BOVINE SERUM ALBUMIN AND OVURELIN HORMONE TO DETERMINE AN EFFECTIVE MEDIA ON IN VITRO MATURATION AND FERTILIZATION OF CATTLE OOCYTES

Abstract

The present experiment has been undertaken to know the effect of hormone and protein supplementation on in vitro maturation and fertilization of cattle oocytes. Ovaries from slaughter were brought to the laboratory and washed in a saline solution at 30℃ for 2 to 3 times. Cumulus oocytes complexes (COCs) were collected by aspiration method and 475 follicles were aspirated out of 631 visible follicles on the surface of both ovaries from CL group (Luteal phase) and without CL (Follicular phase),Where the COCs were classified as normal (Grade A and Grade B) and abnormal (Grade C and Grade D) groups, the highest numbers of normal COCs were found in the ovary without CL (3.11±0.21%). The COCs were matured for 48 hours in TCM-199 basic medium supplemented 10% Bovine Serum Albumin (BSA) and Gonadotropin Hormone (Ovurelin). Cumulus Cell expansion of COCs were studied in TCM-199 cultured media with Ovurelin and 10% BSA. COCs were fertilized with frozen semen after maturation in TCM-199 supplemented with 10% BSA and 10.15µg/ml Ovurelin. The maturation rate is significantly higher at (66.25±2.67%) when 10% BSA and 10.15µg/ml Ovurelin used combined. Similarly, the fertilization rate was significantly higher (P<0.05) and high number of normal pronuclei formation (2PN) (66.25±2.67%) was found when supplemented with 10% BSA and 10.15µg/ml Ovurelin. Thus, it can be concluded that the combined media of 10% BSA and Ovurelin might be used as a good supplement for in vitro maturation and fertilization of this study.