Abstract:
The present experiment has been undertaken to know the effect of hormone and
protein supplementation on in vitro maturation and fertilization of cattle oocytes.
Ovaries from slaughter were brought to the laboratory and washed in a saline solution
at 30℃ for 2 to 3 times. Cumulus oocytes complexes (COCs) were collected by
aspiration method and 475 follicles were aspirated out of 631 visible follicles on the
surface of both ovaries from CL group (Luteal phase) and without CL (Follicular
phase),Where the COCs were classified as normal (Grade A and Grade B) and
abnormal (Grade C and Grade D) groups, the highest numbers of normal COCs were
found in the ovary without CL (3.11±0.21%). The COCs were matured for 48 hours
in TCM-199 basic medium supplemented 10% Bovine Serum Albumin (BSA) and
Gonadotropin Hormone (Ovurelin). Cumulus Cell expansion of COCs were studied in
TCM-199 cultured media with Ovurelin and 10% BSA. COCs were fertilized with
frozen semen after maturation in TCM-199 supplemented with 10% BSA and
10.15µg/ml Ovurelin. The maturation rate is significantly higher at (66.25±2.67%)
when 10% BSA and 10.15µg/ml Ovurelin used combined. Similarly, the fertilization
rate was significantly higher (P<0.05) and high number of normal pronuclei formation
(2PN) (66.25±2.67%) was found when supplemented with 10% BSA and 10.15µg/ml
Ovurelin. Thus, it can be concluded that the combined media of 10% BSA and
Ovurelin might be used as a good supplement for in vitro maturation and fertilization
of this study.
Description:
A THESIS
Submitted to the Department of Animal Nutrition, Genetics and Breeding,
Sher-e-Bangla Agricultural University, Dhaka-1207
in partial fulfillment of the requirements for the degree of
MASTER OF SCIENCE (MS) IN ANIMAL BREEDING AND GENETICS
