Abstract:
Mycotoxins are the toxic substances which are produced as a result of infection
caused by fungi as their secondary metabolite. Major toxin producing fungi are
Fusarium, Penicillium, Aspergillus and Claviceps which produces DON, ZEN, OTA,
FB, AFB, citrinin and patulin. The experiment was carried out in Key Laboratory of
Food Safety and Quality Control, Institute of Food Science and Technology.Chinese
Academy of Agricultural Sciences, Beijing China under completely randomized
design with three replications. The objectives of the experiment were the isolation,
molecular identification and screening of the single bacterial colony which is
responsible for the degradation of Fusarium mycotoxin deoxynivalenol (DON) in
European wheat samples. Wheat samples were mixed using lysogeny broth (LB)
media, PBS media and mineral salt (MM) media for isolation of bacteria using
DONstar immunoaffinity column.Six Bacteria genera namely Pseudomonas spp,
Agrobacterium sp, Zobellella sp, Bacillus sp., Achromobacter sp, Advenella sp and
Cupriavidus sp. were isolated and identified by peR amplifying 16SrDNA gene
fragment.Maximum DON degradation was recorded by mixed bacteria in wheat
samples rather than the isolated single bacteria.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka
in Partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
PLANT PATHOLOGY
