Abstract:
The experiment was conducted in the Disease Diagnostic Laboratory of the
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka
during the period of November 2011-February 2013 to determine the bio-
efficacy of microbial antagonists against foot and root rot pathogen(s) of lentil.
Two fungal genera namely Fusarium oxysporum and Sclerotium rolfsii were
isolated from foot and root rot infected lentil plants. Seven seed treatment
agents, viz., Rhizobium leguminosarum isolate 1, isolate 2, isolate 3, isolate 4,
Pseudomonas fluorescens, Trichoderma harzianum and Control (sterile water)
were used in this experiment. In dual culture method, highest zone of inhibition
of F. oxysporum (57.37%) was measured against R. leguminosarum isolate 3
and isolate 4. In case of S. rolfsii, 80.00% and 31.85% zone of inhibition were
measured' against P. jluorescens and T. harzianum, respectively. In rolled paper
towel method, the highest germination (91.00%) and lowest dead seeds
(9.00%), no abnormal and diseased seedlings were counted from seeds treated
with R leguminosarum isolate I. The maximum vigor index (1390.00) was
observed in P. fluorescens treated seeds. In water agar test tube method, the
highest germination (88.00010) and lowest dead seeds (11.67%) were counted
from R. leguminosannn isolate 4 treated seeds. The maximum number of
normal seedlings (84.56) and minimum number of abnormal seedlings (2.00)
were counted from seeds treated with R. leguminosarum isolate 1 and isolate 2,
respectively. The minimum number of diseased seedlings (0.61) were counted
from T. harzianum treated seeds.
Description:
A Thesis.
Submitted to the Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka,
in partial-fulfillment of the requirements
for the degree of
MASTER OF SCIENCE-
IN
PLANT PATHOLOGY
