Tolerance of Trichoderma, Gliocladium and Sclerotium to Agrochemicals and biocontrol potency of Trichoderma and Gliocladium

Abstract

n-vitro test reveled that Trichoderma harzianum could tolerate Vitavax, Tilt 250EC, Ridornil and Thiovit at 10001Jg/ml, while Gliocladium viride could grow better on PDA containing Ridomil and Thiovit. Trichoderma harzianum and Gliocladium viride could not tolerate even 100IJg/ml of Bavistin. On the other hand, Sclerotium rolfsii could not tolerate Vitavax, Tilt 250EC and Ridomil even at lOOlJglml, but Bavistin and Thiovit reduced the growth of Sclerotium rolfsii by 86.18% and 75.64%, respectively at 1000~glm) and IOOJ.1g1ml.Out of the nutrient elements tested, N, K, S and Mn reduced the growth of Trichoderma harzianum while slight induction in growth was recorded by P and Cu. Except P, the tested metal salts reduced the growth of Gliocladium vir ide, where higher concentration of Imetal salts in medium resulted- higher inhibition of growth. Nutrients showed inhibition of growth of Sclerotium rolfsii at increasing the cone. in medium. The antagonists were found potential against Sclerotium rolfsii. Seed treatment with antagonists resulted upto 21.61%, 53.5%, 26.64% and 48.43% increase in germination in mungbean, blackgram, pigeon pea and tomato, respectively and showed good effect on seed borne mycoflora. Moreover, significant growth enhancement of mungbean, blackgram and tomato have been achiev.ed by treating seeds with antagonists.