Abstract:
An experiment was conducted at North Bengal Agro Farms Ltd.,
Thakurgaon, Bangladesh, during the period of January to September 2020
for plantlet regeneration of potato using different new composition of stock
solution-1. Three chemicals viz. chemi-i, chemi-ii and chemi-iii were used in
stock solution-1 as an alternate of ammonium nitrate. Eight different
treatments were designed with those chemicals including Murashige and
Skoog (1962) culture media as a check treatment (T
). Two potato varieties
viz. Diamant and Asterix were used for in vitro regeneration. The Diamant
variety showed the best regeneration potentiality in the T
3
, which consists of
the chemi-iii. The check treatment (T
3
) showed 2
nd
7
highest performance for
all the parameters under studied. The Asterix variety gave the best
performance in the check treatment (T
3
) and the 2
nd
highest result was found
in the T
. Different abnormalities were noticed in respect of media and
plantlet regeneration. Brown, deep brown, off white and contaminated colour
was found in the treatments T
4
1
, T
2
, T
5
, T
6
and T
. Plantlet regeneration from
those treatments showed aberrancy and it has stunted growth, dwarf size,
small leaf formation, black layer at the root tips and finally plantlet died
within 4
th
week of regeneration. The T
7
8
treatment showed yellow colour and
the plantlet regenerated from this treatment were thin, tall with less number
of roots. The plantlet regenerated from check treatment (T
) showed good
growth and development throughout the experimental period. Finally, it can
be concluded that, the chemi-iii, can be used as a component of stock
solution-1 which will be a good alternate of ammonium nitrate.
