Abstract:
The present research was carried out in Biotechnology Laboratory of the Department
of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar,
Dhaka -1207 from the period of July 2020 to June 2021 for in vitro rapid regeneration
of Gladiolus. The healthy, disease free cormel of 1.5 cm- 2.0 cm were used as
explants, was sterilized by 0.1% Bavistin followed by 0.1% HgCl2 mixing with few
drops Tween-20. The explant were inoculated in MS media supplemented with
different combination of Benzyl Adenene (1.0, 2.0, 3.0, 4.0, 5.0 mg/L) and
Naphthalene Acetic Acid (0.5, 1.0, 1.5, 2.0 mg/L). The minimum days (11.00) to
shoot (73.80%) induction with the highest percent of shoots induction were achieved
in 4.0 mg/L BA. The highest number of shoot (1.60, 2.20, 2.40) and maximum shoot
length (0.74 cm, 1.18 cm and 1.32 cm) were also observed in 4.0 mg/L BA and at 21,
35 and 45 Days After Induction (DAI) respectively. The combine effect of auxins and
cytokinins were also investigated where 4.0 mg/L BA + 0.5 mg/L NAA performed the
best incase highest frequency of shoot induction (74.80%) with minimum days
(10.60) and gave the maximum shoot number (1.80, 2.60, 2.80) and shoot length (1.40
cm, 2.32cm, 2.82 cm) at 21, 35 and 42 DAI respectively. Moreover, the treatment 4.0
mg/L BA + 2.0 mg/L NAA showed the highest frequency (79.80%) of root induction
with minimum days (8.40) and gave the maximum number (4.20, 6.00, 7.20) of root
with optimum root length (1.02 cm, 1.26 cm, 1.34 cm) at 21, 35 and 42 DAI. In
shaded condition the survival rate of plantlet was 83.33% and in open atmospheric
condition the survival rate was 76%. Overall, a reliable and feasible protocol had been
developed for in vitro multiplication of Gladiolus which can be a useful tool for tissue
culture of Gladiolus
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfilment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTCHNOLOGY
