Abstract:
Cattle and buffalo play a vital role in the livestock production sector in
Bangladesh, but their production is problematic due to some reproductive
difficulties. To address this issue, In Vitro Embryo Production (IVP) would
be the appropriate option of animal production. Thus, in this study, the
qualitative and quantitative features of the ovary and oocytes of these two
species were compared in order to gain a full understanding of them and to
pick the optimal oocytes for IVP. The ovaries were collected from the local
slaughter houses, aspirated the follicular fluid, and classified the oocytes.
The nuclear diameter of oocytes was measured to know the change occurred
due to maturation. The ovary of cow and buffalo differed significantly
(p<0.01) in weight (1.75 vs. 4.83 g), length (1.53 vs. 2.29 cm), width (0.89
vs. 1.57 cm), and depth (0.63 vs. 1.03 cm). Normal oocytes per ovary were
1.19 in cattle and 1.50 in Buffalo, while abnormal oocytes were 0.96 in
cattle and 1.13 in Buffalo. Also, a significant difference (p<0.05) was
observed between the oocytes’ nuclear diameter (cattle 85.64 ± 8.16 µm vs.
buffalo 99.92±8.65 µm) and before & after maturation of oocytes within the
same species. It may be concluded that, the buffalo ovary was more
prominent in size and heavier in weight than the cattle ovary. Besides, any
type of ovaries - irrespective of the ovary's position and CL status - can be a
good source of potential oocytes.
Description:
A Thesis
Submitted to the Department of Animal
Production and Management, Faculty of
Animal Science and Veterinary Medicine,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements for the
degree of
MASTER OF SCIENCE
IN
ANIMAL SCIENCE
Semester: January - June, 2021
