Abstract:
The research work was conducted in the Laboratory of Biotechnology of the Department of
Biotechnology, Sher-e-Bangla Agricultural University, from the period of July 2015 to June,
2016 to observe the response of different plant growth regulators on in vitro regeneration of
garlic. The disease free, healthy and sterilized basal part of clove of 2-3 cm length were used
as explants. Sterilization was done with 70% ethanol, 0.5% HgCl
2
along with few drops of
Tween-20. Explants were placed in MS medium supplemented with the Kinetin (KIN), alone
and in combination with 2, 4-Dichlorophenoxy acetic acid (2, 4-D). The highest shoot
regeneration percentage (100%) was observed by 3.0 mg/L of KIN alone and by wide range
of KIN in combination with 2, 4-D (i.e. all combination of 2.5, 3.0 mg/L KIN with 1.0, 1.5,
2.0, 2.5 mg/L 2, 4-D) . The highest shoot length (34.50 cm) was observed after 3 weeks of
initiation in 3.0 mg/L KIN treatment. Combined doses of KIN and 2, 4-D showed 1 shoot
after 3 weeks of initiation. Number of leaves did not exceed 3 and it was noticed through all
combination of 2.0, 2.5, 3.0 mg/L KIN with 1.0, 1.5, 2.0, 2.5 mg/L of 2, 4-D. The highest
root induction (100%) was observed by 2.5 mg/L KIN + (1.0, 1.5) mg/L 2, 4-D. It took
minimum 3 days to induce root. The average and the highest length of root (3.5 cm and 2.93
cm respectively) was noticed by application of 3 mg/L KIN and 1.0 mg/L 2, 4-D. Maximum
number of roots (10.67) were developed by 3.0 mg/L KIN+ 1.5 mg/L 2, 4-D. After
transferring the plantlets in the field condition, 86% survival plants were recorded. Finally, in
vitro regeneration of garlic was proved to be less time consuming. Moderate doses of
hormones showed satisfactory responses in shoot proliferation and root induction which
made the tool cost effective as well.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
SEMESTER: JANUARY-JUNE, 2016