Abstract:
An experiment was carried out with twenty five local and two wild relatives (Solanum
sisymbriifolium and S. torvum) of eggplant at the research field and in the
Biotechnology Laboratory of Sher-e-Bangla Agricultural University, Dhaka,
Bangladesh, during October 2015 to June 2016 to investigate the phenotypic
characterization and molecular diversity analysis of different local eggplant
germplasm and its wild relatives. The morphological study revealed diversified
characters among all the genotypes. Variations were observed in color of leaf vein,
stem as well as fruit size and shape. Maximum yield per plant was recorded in Salta
Begun and it was 9.8 kg. The minimum yield per plant was observed in both the wild
species (3.8 kg) as they produced very small fruits. Correlation of yield contributing
traits separated all the genotypes into four clusters (A, B, C, and D). Among them
cluster D had five genotypes together with the wild species indicated their relatedness.
Five well-known SSR primers were used for the molecular characterization of the
genotypes. Ten alleles, ranged from 1 to 3 alleles per locus and an average of 2.0 were
detected. Among five primers three were able to produce polymorphic bands and the
total number of polymorphic bands was five. The highest (2) number of bands was
observed in SSR primers EPSSR82 and smSSR01. The Polymorphism Information
Content (PIC) of SSR markers ranged from 0.37 (smSSR01) to 0.67 (EPSSRR82)
with an average value of PIC = 0.54. Gene diversity ranges from 0.49 (smSSR01) to
0.72 (EPSSR82), with an average value of 0.61. The value of pair-wise comparisons
of Nei‟s (1972) genetic distance (D) and identity between varieties was computed
from combined data for the five primers, ranged from 0.20 to 1.00, with an average
value of 0.60. Multivariate cluster analysis on the basis of Nei‟s genet ic distance and
identity revealed that within those 27 genotypes there surely do exist considerable
diversity. UPGMA method separated the segregation of 27 genotypes into two major
clusters (I and II). From the clusters, wild species Solanum torvum belonged to the
sub-cluster (IIb), that revealed its distinct variation from the others. On the other hand,
wild species Solanum sisymbriifolium showed a close relatedness by forming the
same cluster together with thirteen local eggplant genotypes. This experiment brought
a great opportunity for eggplant improvement as the local genotypes had a close
relation with the wild species.
Description:
A Thesis Submitted to
The Department of Biotechnology, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207
In partial fulfillment of the requirements
For the degree
Of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
SEMESTER: JANUARY-JUNE, 2016