Abstract:
An experiment was carried out in Biotechnology Laboratory of the Department of
Biotechnology, Sher-e-Bangla Agricultural University (SAU), Dhaka, Bangladesh
to develop low cost and rapid genomic DNA isolation technique and to study the
DNA fingerprinting and genetic diversity of nine garlic genotypes. Conventional
CTAB/SDS method of DNA extraction required more time and cost. In contrast,
the newly developed method to low cost and very fast method for extraction of
genomic DNA in bulb crops. The garlic germplasms were BARI-G1, GC001,
GC008, GC0012, GC0013, GC0017, GC0024, GC0027 and GC0028. Genomic
DNA was extracted in both methods to perform PCR reaction with seven RAPD
primers. Sum of 53 distinct and differential amplified DNA bands were generated
from PCR amplification. Out of them, 40 bands (75.88%) were polymorphic and
13 bands (24.12%) were monomorphic. The Nei’s genetic identity among 9 garlic
genotypes ranged from 0.8053 to 0.9966 and genetic distance ranged from 0.0053
to 0.2166. The UPGMA Dendrogram segregated the 9 garlic genotypes into two
main clusters. The first cluster contained 1 genotypes and the second cluster had 8
genotypes. The Dendrogram also indicated that BARI-G1 vs GC0027 varietal pair
showed highest Nei’s genetic distance (0.2166) and GC0012 vs GC0017 varietal
pair showed lowest genetic distance (0.0053). The study revealed a significant
amount of relationship and genetic diversity among the studied 9 garlic genotypes.
The RAPD markers were found to be useful tool for molecular characterization
and polymorphism study in garlic genotypes.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka
In partial fulfillment of the requirements
For the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
SEMESTER: January - June, 2016
