Abstract:
An experiment was undertaken at the Biotechnology Laboratory, Department
of Biotechnology, Sher-e-Bangla Agricultural University (SAU), Dhaka,
Bangladesh during the period of January, 2015 to June, 2016 to identify a new
chemical for the substitute of explosive chemical Ammonium nitrate
(NH
4
NO
) in MS media composition for in vitro regeneration of potato
(Solanum tuberosum L.). Three potato varieties viz Diamant, Cardinal and
Asterix were used as an experimental materials. The new chemical was denoted
as β chemical for the privacy of the experimental findings. Different
concentrations (1, 5. 10, 15 and 20 gmL
3
-1
) of β chemical were used for the
preparation of stock solution- A, which was finally used for MS media
preparation. It was noticed that in vitro regeneration of potato has been
successfully done with new composition of β chemical in MS media
preparation. The shoot length, node number, number of leaf, root length were
highest in 5 gmL
-1
of β chemical at 14, 21 and 30 days after sub-culture (DAS).
Although, in some parameters liquid MS standard dose (16.5 gmL
) gave
maximum result but it was statistically non significant with 5 gmL
of β
chemical. All the plantlets were died at higher concentration (15 gmL
and 20
gmL
-1
) of β chemical. It may be due to toxic effect of the chemical. The variety
Diamant showed best performance in all the traits under studied. It is reveal
that ready made MS powder, Liquid MS standard dose and 1 gmL
of β
chemical showed comparatively similar result on most of the parameters under
studied. Among the varieties, the Asterix variety showed minimal performance
in most of the phenotypic characters under studied. In all the case, Diamant
variety showed best performance for all the phenotypic characters under
studied. The newly used β chemical is cheap, non explosive and environmental
friendly which can be an alternative of destructive chemical Ammonium nitrate
-1
-1
-1
-1 (NH
4
NO
) for MS media preparation.
3
Description:
A Thesis
Submitted to the Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
SEMESTER: JANUARY-JUNE, 2016
