Abstract:
ABSTRACT
This study was conducted to investigate the E. coli and Salmonella from drinking water
in SAU campus and its surrounding areas from January to August 2021. A total of 60
samples were collected from different halls, faculties and neighboring market areas of
the SAU campus. Bacteria was identified observing the growth properties in different
media, staining properties, microscopic observation and biochemical test. The
characteristics colonies of E. coli were red to bright pink colored in MA agar & greenish
red colored with faint metallic sheen in EMB agar. The characteristics colonies of
Salmonella were black colored in SS agar & gray colored in EMB agar. Both bacteria
observed in rod shaped, gram negative, single or paired arranged under microscope. In
biochemical test both bacteria showed Catalase test, MR test positive and VP test
negative. In case of Indole test E. coli showed positive but Salmonella showed negative
indication. The higher prevalence (66.67%) was in Krishokrotno Sheikh Hasina Hall &
Sheikh Sayera Khatun Hall. For faculties, the lower (33.33%) prevalence was in Sheikh
Kamal Bhaban and higher (66.67%) was in Agriculture Faculty Bhaban. All samples
collected from college gate street food shop were positive. The prevalence of E. coli &
Salmonella in this study areas were 20% and 33%. The mixed contamination was 5%.
So, direct consumption of water should not safe for us. It can cause serious health
hazard.
Description:
A Thesis
Submitted to the Faculty of Animal Science and Veterinary Medicine Sher-e-Bangla Agricultural University, Dhaka-1207, In Partial Fulfillment
of the Requirements for the Degree of
MASTER OF SCIENCE (MS) IN MICROBIOLOGY
DEPARTMENT OF MICROBIOLOGY AND PARASITOLOGY
Semester: Jan-Jun, 2019