Abstract:
ABSTRACT
An experiment was conducted in pot and laboratory condition under the Department
of Plant Pathology of Sher-e-Bangla Agricultural University, Dhaka-1207 during
2020-2021.The pot experiment was carried out in a Completely Randomized Design
(CRD) with ten replications. RT-PCR test was done in Molecular Biology and Plant
Virology Laboratory. In this experiment ten varieties/lines viz., Tain 927, Jonaki
707, Rocket 55, Konok 51, Kohinoor, Line A.S, Line A, Line 984, Khoi Bhutta and
Sweet corn were selected for conducting the experiment. Among the ten tested
varieties/lines, Tain-927, Rocket-55 and Line A were showed tolerant. Variety Khoi
bhutta was showed highly susceptible against Maize Lethal Necrosis Disease
(MLND). Remaining varieties were showed susceptible to moderately susceptible.
Molecular study was also performed for the detection of targeted maize viruses that
cause MLN disease. From molecular study through RT-PCR test, it was revealed
that results obtained on the basis of biological properties. All the selected
varieties/lines gave the positive results in RT-PCR test against Maize Chlorotic
Mottle Virus but showed negative result against Sugarcane Mosaic Virus. Different
morpho-physiological, yield and yield contributing characters were also studied.
Among the selected varieties, the maximum height of plant was obtained in the
variety Tain-927 and minimum was obtained in the variety Khoi bhutta. The highest
chlorophyll content was recorded in the variety Konok-51 and the lowest was
recorded in the variety Kohinoor. The maximum number of fruits fruit weight and
length was obtained from variety Tain-927 and the minimum was obtained from
variety Khoi bhutta. Both disease incidence and severity show negative relation with
plant height and chlorophyll content. From the study, it may be concluded that maize
viruses were transmitted through hybrid seeds.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfilment of the requirements
for the degree of
MASTER OF SCIENCE
IN
PLANT PATHOLOGY
SEMESTER: JUNE, 2021
