MICROPROPAGATION OF BLACK PEPPER (Piper nigrum L.)

Abstract

The present study was undertaken in the Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e- Bangla Nagar, Dhaka-1207, during the period from January to September, 2020 to evaluate the effect of Benzyl adenine (BA) Indole-3-acetic acid (IBA) on in vitro regeneration of Black pepper. The nodal segments of Black pepper were used as explants which were inoculated in MS media supplemented with different conc. (0.5, 1.0, 1.5 and 2.0 mg/L) of BA and IBA (0.5, 1.0, 1.5 and 2.0 mg/L) either alone or in combination of BA (1.0, 1.5, 2.0 and 2.5 mg/L) and IBA (1.0, 1.5, 2.0 and 2.5 mg/L). The experiments were arranged in Completely Randomized Design (CRD) with five replications. The maximum shoot induction was obtained with 2.0 mg/L BA.The highest number of leaves were recorded in the treatment 2.0 mg/L BA at 8 WAI. The maximum number of root was regenerated in the treatment 2.0 mg/L IBA. The combination of both the hormone 2.0 mg/L BA + 2.0 mg/L IBA showed best result for shoot induction, shoot length and also for total number of leaf. Maximum percentage of root induction and root length also found in the same treatment. Regenerated plantlets survive 46.67% in shade house and 57.14% in open atmosphere. Therefore, an efficient protocol has been developed for in vitro regeneration of Black pepper which can be used for large scale production of Black pepper.