Abstract:
The present study was undertaken in the Biotechnology Laboratory of the
Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-
Bangla Nagar, Dhaka-1207, during the period from January to September, 2020
to evaluate the effect of Benzyl adenine (BA) Indole-3-acetic acid (IBA) on in
vitro regeneration of Black pepper. The nodal segments of Black pepper were
used as explants which were inoculated in MS media supplemented with different
conc. (0.5, 1.0, 1.5 and 2.0 mg/L) of BA and IBA (0.5, 1.0, 1.5 and 2.0 mg/L)
either alone or in combination of BA (1.0, 1.5, 2.0 and 2.5 mg/L) and IBA (1.0,
1.5, 2.0 and 2.5 mg/L). The experiments were arranged in Completely
Randomized Design (CRD) with five replications. The maximum shoot induction
was obtained with 2.0 mg/L BA.The highest number of leaves were recorded in
the treatment 2.0 mg/L BA at 8 WAI. The maximum number of root was
regenerated in the treatment 2.0 mg/L IBA. The combination of both the hormone
2.0 mg/L BA + 2.0 mg/L IBA showed best result for shoot induction, shoot length
and also for total number of leaf. Maximum percentage of root induction and root
length also found in the same treatment. Regenerated plantlets survive 46.67% in
shade house and 57.14% in open atmosphere. Therefore, an efficient protocol has
been developed for in vitro regeneration of Black pepper which can be used for
large scale production of Black pepper.
Description:
A Thesis
Submitted to the Dept. of Biotechnology
Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfilment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
