Abstract:
A series set of test of this experiment was carried out to evaluate the seed health and
quality status of ten selected vegetables viz. amaranth, Indian spinach, bottle gourd,
sweet gourd, snake gourd, okra, bitter gourd, cucumber, brinjal and country bean during
November 2015 to April 2016 at Seed Health Laboratory of Department of Plant
Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. Untreated and
unknown grower’s bulk vegetable’s loose seeds were collected from three different
seed sources of Rangpur sadar viz. New Bangla Seeds; Islam Seeds and Rafiq Traders.
Seed health study was conducted by following inspection of dry seed, blotter method,
rolled paper towel method and growing on test. In inspection of dry seed method,
pathogenic structures, mycelium (white and cottony) and acervuli were recorded. The
observed physical abnormalities viz. deformed, shrinkage, swelling, spotted and
undersized seeds were recorded. In most cases, the highest percentage of pure seed was
recorded in seeds collected from Rafiq Traders followed by New Bangla Seeds. It was
exposed that seeds of selected vegetables yielded six fungal species in blotter method.
The identified fungi were Aspergillus flavus, Aspergillus niger, Fusarium spp.,
Alternaria spp.,
Chaetomium spp. and Rhizopus sp. Seed infection of an unidentified
bacterium was also recorded. The highest total seed borne fungal infection was found in
the seeds of New Bangla Seeds. In rolled paper towel methods, seed germination varied
from 70-94%, normal seedlings were 56-84%, abnormal seedlings were 5-18%, rotten
seeds were 4-15%, hard or ungerminated seeds were 2-18% and vigor index was 677.3
to 2747. In growing on test method, seed germination varied from 65-99%, normal
seedlings were 51-90%, abnormal seedlings were 8-19%, rotten seed were 1-15%, hard
seeds were 1-24% and vigor index was 666.6 to 2747. In both methods, the highest seed
germination and seedling vigor was recorded in amaranth and country bean of New
Bangla Seeds, respectively. The overall health status and quality of bulk vegetable’s
loose seeds were not up to the mark. Most of the seed-borne pathogens reduced the
germination and produced diseased seedlings. Thus, seed treatment is suggested for the
farmers/growers before sowing vegetable’s loose seeds.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka
in Partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
PLANT PATHOLOGY
SEMESTER: DECEMBER, 2015