Abstract:
Incidence of potato virus Y in the three selected potato growing areas of Bangladesh, viz.
Savar, Manikgonj and Munshiganj were investigated from field samples. Overall, three
upazilla (sub-station) from each district (which were at least 20 km apart) were selected
for samples collection and disease incidence monitoring. Collected samples were storage
at -20°C and analyzed in Molecular Biology and Plant Virology lab, Central Research
Laboratory of Sher-e-Bangla Agricultural University (SAU), Dhaka. In the all
investigated areas, PVY appeared in severe to moderate level and their incidence was 317%.
The sources of potato seed tubers were studied and observed that in most of the
cases farmers are continuously cultivating same field and used their own seed tubers that
was kept in cold storage condition. The frequency of the storage at cold storage was
67.6% which was the highest among the all sources. In this study, PVY was detected
through reverse transcriptase polymerase chain reaction (RT-PCR). A 480 bp amplicon of
PVY- coat protein (CP) gene was amplified and the nucleotide sequences was also
amplified in this study.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
MASTER OF SCIENCE
IN
PLANT PATHOLOGY
