Abstract:
In vitro maturation (IVM) of oocytes provide an excellent opportunity for
cheap and abundant embryos for carrying out animal improvement. With the
aim to study the oocyte quality in different seasons of Black Bengal goat, both
right and left ovaries were collected from the slaughter houses. For each of the
specimens, gross parameters such as right, left, corpus luteum (CL)-present
and -absent group and evaluated on the basis of weight (g), length (cm), width
(cm), follicles aspirated and number and state of cumulus-oocyte-complexes
(COCs), normal COCs and abnormal COCs, the effect of season on cumulus
oocyte complexes (COCs) of goat oocytes. Our study revealed that the average
number of follicles in both left and right ovaries were higher in summer
(12.50; 10.48) than in winter season (8.08; 8.21). Both right and left ovaries
are great source for large number of oocyte and without-CL ovaries are
considered as suitable source to supply quality oocyte for in vitro embryo
production of goat. In different corpus luteum-present ovaries total number of
follicles were distinctly higher in summer season (9.00±1.00) compared to that
of winter season (6.50±1.84). In different corpus luteum-absent ovaries total
number of follicles were distinctly higher in summer and winter season (13.52;
11.84) compared to that of CL present ovary in summer and winter season
(9.00; 6.50). The oocyte recovery rate (2.14) was higher in summer than in
(1.97) winter season. The results obtained in the present study showed that
recovery rate of grade A and B oocytes were higher in summer than that of in
winter and recovery rate of grade C and D were higher in winter than that of
in summer. In the present study a significantly greater number of oocytes per
ovary were recovered from ovaries without a corpus luteum (2.23; 2.02) than
from ovaries with a corpus luteum(1.50; 1.50). Greater number of oocytes per
ovary were recovered from ovaries with and without a corpus luteum (1.50;
2.23) in summer than from ovaries with and without a corpus luteum (1.50;
2.02) in winter. So the summer was the best season for recovery of COCs
which have a worthy competence to be matured in vitro.
Description:
A Thesis
Submitted to the Department of Animal Production and Management
Sher-e-Bangla Agricultural University, Dhaka
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
ANIMAL SCIENCE
