ISOLATION, IDENTIFICATION & MOLECULAR CHARACTERIZATION OF Escherichia coli AND Salmonella spp. FROM FECAL MATERIALS OF POPULAR PET BIRDS

Abstract

Recently, rearing of pet birds have increased with the increased socio-economic condition of the citizen. These pets population may contribute many zoonotic bacteria to public health. Salmonella spp. and E. coli are considered as the most common zoonoses in the world, causing important losses to public health. Considering these significances, this study assessed the occurrence of Salmonella spp. and Escherichia coli in caged Pigeon, Budgerigar & Lovebird. A total of 45 fecal samples were collected as environmental sample from these 3 different species of birds from pet shops of Katabon, Dhaka. Twenty samples from Pigeon (P-1 to P-20), Fifteen samples from Budgerigar (B-1 to B-15) & Ten samples from Lovebird (L-1 to L-10) were collected. Birds with positive cases of E. coli were found 22 (48.8%) & positive cases of Salmonella spp. were found 8 (17.7%). Rest 15 (33.33%) samples were unidentified. The occurrence of E. coli were 7 (35%), 11 (73.33%) and 4 (40%) in case of pigeon, budgerigar and lovebird respectively. The occurrence of Salmonella spp. in pigeon was 40%. No isolates of Salmonella spp. were found in budgerigar & lovebird. The antibiotic study revealed that all the isolates of E. coli were 100% sensitive to Ciprofloxacin, Norfloxacin, Amikacin, Azithromycin & Gentamicin. All the isolates of E. coli were 100% resistant to Tetracyclin, Ampicillin & Colistin sulphate. All the isolates of Salmonella spp. were 100% susceptible to Amoxicillin, Amikacin & Gentamicin and showed 100% resistance to Tetracycline, Trimethoprim & Colistin sulphate. These results analyze that birds that were analyzed may carry and spread these enterobacteria, and preventive measures for human exposure should be determined, as these microorganisms are public health concerns.