Abstract:
A study was conducted at Plant Pathology Laborartory, Sher-e-Bangla
Agricultural University, Dhaka-1207 during February 2019 to March 2020 to
isolate, identify, and characterize the causal agents responsible for post-harvest
diseases of fruits. Apple, pear, orange and papaya were used as samples and
collected from different fruit markets in Dhaka city. Post-harvest fungi were
isolated on PDA medium following blotter paper method. Four different genera
of fungi were isolated and identified viz. Aspergillus spp., Penicillium spp.,
Fusarium spp. and Sclerotium sp. Of these, Aspergillus spp. was common in
apple, pear, orange and papaya. Pathogenicity test showed all isolated fungi
pathogenic to their respective host. Effect of different media, temperature and
packaging on growth and development of isolated fungi was studied. Study was
carried out by completely randomized design with three replications. All
isolates showed variation in myceial growth, surface colour, colony colour,
colony shape and colony texture. On PDA media, the highest radial mycelial
growth (88.19 mm) was recorded in Sclerotium rolfsii (Apple) and lowest
(23.62 mm) in Penicillium digitatum (pear) at 10
th
DAI. At 30℃ temperature,
the highest radial mycelial growth (90.00mm) was recorded in Sclerotium
rolfsii (Apple) and lowest (23.53 mm) Penicillium digitatum (orange) at 10
DAI. In paper packaging, long days were required for deterioration in case of
apple (60 days) and shortest days were required for deterioration in case of
papaya (6days). Sclerotium rolfsii (Apple) showed best performance in radial
mycelia growth observation (media and temperature) and apple showed best
performance in all packaging observation. In this study, PDA medium, 30°C
temperature showed best results for mycelial growth of isolated pathogen and
paper bag showed best results for packaging of fruits at room temperature.
Description:
A Thesis
Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University,
Dhaka- 1207, in partial fulfillment of the requirements for the degree of
MASTER OF SCIENCE
IN
PLANT PATHOLOGY