Abstract:
An experiment was carried out to analysis the genetic diversity of 24 Bangladeshi T. Aman rice
(Oryza sativa L.) germplasm using morphological traits and molecular markers. The experiment
was conducted at Genetic Resources and Seed Division (GRSD), Bangladesh Rice Research
Institute (BRRI), Gazipur, during the period of July to December 2019. Observed variables
included qualitative and quantitative traits. A total of 10 qualitative and 10 quantitative
characters were studied. Qualitative characters were descriptively analyzed and showed variation
in different accessions. Quantitative data were also subjected to cluster analysis which showed
total three cluster and many more sub-clusters. So the plant morphological traits exhibited more
variation among the germplasm tested. A set of sixteen SSR markers was used for molecular
diversity study which resulted 66 alleles with an average of allele number was 4.13. The
polymorphic information content (PIC) values ranged from 0.08 (RM455) to 0.80 (RM206) with
an average of 0.48. The highest PIC value (0.80) was obtained from RM206. The PIC value
revealed that RM206 was the best marker for 24 genotypes tested. The value of pair-wise
comparisons of Nei’s genetic distance among 24 rice germplasm was computed from combined
data for the 16 primers, ranged from 0.1250 to 0.9375 with an average of 0.5247. The cluster
analysis based on UPGMA system grouped 24 germplasm into three main clusters and total six
sub-cluster. Both the dendrogram showed diverse variation. Evaluation of morpho-molecular
characters demonstrated that aman rice germplasm under the present study possessed a high
genetic diversity which may be used for rice breeding program.
Description:
A Thesis submitted to
The Department of Biotechnology, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207
In partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
