Abstract:
An experiment was undertaken at the Biotechnology Laboratory,
Department of Biotechnology, Sher-e-Bangla Agricultural University
(SAU), Dhaka, Bangladesh during the period of January, 2018 to January,
2019 to identify a new chemical for the substitute of explosive chemical
Ammonium nitrate (NH
4
NO
) in MS media composition for in vitro
regeneration of potato (Solanum tuberosum L.). One potato variety Asterix
was used as an experimental materials. The new chemical was denoted as
α-chemical for the privacy of the experimental findings. MS powder, MS
liquid standard dose, different concentrations (1, 5, and 10 gmL
3
) of α
chemical which have twice dose of other component in stock solution-I for
the preparation of tissue culture media were used as treatment. It was
noticed that in vitro regeneration of potato has been successfully done with
α-chemical in new composition of tissue culture media. The shoot length,
node number, number of leaf, root length were highest in MS powder (T
)
at 14, 21 and 28 days after inoculation (DAI). Although, in some
parameters MS powder (T
) gave maximum result but it was statistically
non-significant with 5gm of α- chemical/litre in stock solution-I having
twice dose of other components in new media composition. The treatment
5gm of α-chemical/litre in stock solution- I having twice dose of other
component showed average performance in all the traits under studied. MS
powder, Liquid MS standard dose and 1 gmL
0
-1
in stock solution- I twice
dose of α chemical showed comparatively similar result on most of the
parameters under studied. The newly used α-chemical is cheap, nonexplosive
and environmental friendly which can be an alternative of
destructive chemical Ammonium nitrate (NH
4
NO
) for tissue culture media
preparation.
