ISOLATION, IDENTIFICATION & ANTIBIOGRAM PROFILING OF STAPHYLOCOCCUS AUREUS FROM FRESH COW MILK SAMPLE

Abstract

Staphylococcus aureus is an important pathogen causing a wide spectrum of diseases in both humans & animals. S. aureus produces a variety of virulence factors that are liable for sub- clinical & persistent intra-mammary infections like mastitis. This research was suspected to isolation, identification & characterization of S. aureus causing bovine mastitis in cattle. The bacteria was isolated, identified & characterized by cultural, staining, morphological, & biochemical. The 12 isolates produced β hemolysis on 5% sheep blood agar & fermented on mannitol salt agar & aslo produced yellow colonies. In Gram staining, the organism found as gram positive, grapes like clusters formed by cocci. The isolates showed catalase & coagulase positive that confirmed as S. aureus. The antimicrobial susceptibility pattern of S. aureus isolates were investigated by disc diffusion method. In this study 42 fresh milk samples were obtained from three different points of Savar, Dhaka, Bangladesh like Savar Hat Area, Shimulia Hat Area & Bagbari Bazar Area & 12 samples yielded positive result for S. aureus, given an overall prevalence of 28.57%. The antibiotic susceptibility profile of the 12 S. aureus isolates revealed that the majority of the isolates were resistant against penicillin, erythromycin & amoxicillin. The isolates showed 100% resistant to penicillin & amoxicillin, while ciprofloxacin, oxicillin, cloxacillin & neomycin were found the most effective drug for the treatment of S. aureus.